VISTA Polyclonal antibody, PBS Only

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  WB analysis using 31619-1-AP (same clone as 31619-1-PBS)

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 31619-1-AP (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31619-1-AP (VISTA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 31619-1-AP (same clone as 31619-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (31619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31619-1-PBS in a different storage buffer formulation.

VISTA Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 31619-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

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Product Information

31619-1-PBS targets VISTA in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H7M9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.