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VPRBP Polyclonal antibody
VPRBP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11612-1-AP
Validation Data Gallery
|Positive WB detected in||DU 145 cells, HeLa cells, mouse testis tissue, PC-3 cells, HEK-293 cells, K-562 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human prostate cancer tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
11612-1-AP targets VPRBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VPRBP fusion protein Ag2184|
|Full Name||Vpr (HIV-1) binding protein|
|Calculated molecular weight||1506 aa, 169 kDa|
|Observed molecular weight||169 kDa|
|GenBank accession number||BC022792|
|Gene ID (NCBI)||9730|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).
|Product Specific Protocols|
|WB protocol for VPRBP antibody 11612-1-AP||Download protocol|
|IHC protocol for VPRBP antibody 11612-1-AP||Download protocol|
|IF protocol for VPRBP antibody 11612-1-AP||Download protocol|
|IP protocol for VPRBP antibody 11612-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases.
Vpr Targets TET2 for Degradation by CRL4VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.
Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.
Cell Host Microbe
Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production.
Cell Host Microbe
Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Nikhil (Verified Customer) (09-17-2019)
Very good for both IP and western blot.