VPS37A Monoclonal antibody

VPS37A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F9A4

Cat no : 66327-1-Ig

Synonyms

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

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Validation Data Gallery

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  WB analysis of SW 1990 using 66327-1-Ig

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66327-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 66327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 66327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66327-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: SW 1990 cells, MCF-7 cells
• Positive IHC detected in: human kidney tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

66327-1-Ig targets VPS37A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VPS37A fusion protein Ag19108
• Full Name: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC022363
• Gene Symbol: VPS37A
• Gene ID (NCBI): 137492
• RRID: AB_2881708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

Publications

Species	Application	Title
human	IHC	Front Endocrinol (Lausanne); Stratification of diabetic kidney diseases via data-independent acquisition proteomics–based analysis of human kidney tissue specimens; Authors - Qinghua Huang