VPS52 Polyclonal antibody

VPS52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11662-2-AP

Synonyms

Vacuolar protein sorting-associated protein 52 homolog, SACM2L, SAC2 suppressor of actin mutations 2-like protein

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Validation Data Gallery

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  WB analysis of HepG2 using 11662-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 11662-2-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11662-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 11662-2-AP

  IP result of anti-VPS52 (IP:11662-2-AP, 3ug; Detection:11662-2-AP 1:1000) with mouse skin tissue lysate 3200ug.

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  IHC staining of human gliomas using 11662-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11662-2-AP (VPS52 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11662-2-AP

  Immunofluorescent analysis of HepG2 cells using 11662-2-AP (VPS52 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, COLO 320 cells, mouse skin tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11662-2-AP targets VPS52 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS52 fusion protein Ag2180
• Full Name: vacuolar protein sorting 52 homolog (S. cerevisiae)
• Calculated Molecular Weight: 723 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC032108
• Gene Symbol: VPS52
• Gene ID (NCBI): 6293
• RRID: AB_2241614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).

Publications

Species	Application	Title
mouse	WB	Cell Rep; The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.; Authors - Jian Wei
human	IHC, WB	J Mol Med (Berl); VPS52 induces apoptosis via cathepsin D in gastric cancer.; Authors - Jian Zhang