VILIP1 Monoclonal antibody

VILIP1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67134-1-Ig

Clone No. 2C6C3

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

VILIP 1, VILIP-1, VSNL1, 2C6C3, Hippocalcin like protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of human brain using 67134-1-Ig
human brain tissue were subjected to SDS PAGE followed by western blot with 67134-1-Ig (VSNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat brain using 67134-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 67134-1-Ig (VSNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 67134-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67134-1-Ig (VSNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 67134-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67134-1-Ig (VSNL1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 67134-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67134-1-Ig (VSNL1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67134-1-Ig targets VILIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag4923 Product name: Recombinant human VSNL1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-191 aa of BC022012 Sequence: MGKQNSKLAPEVMEDLVKSTEFNEHELKQWYKGFLKDCPSGRLNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQRVDKIFSKMDKNKDDQITLDEFKEAAKSDPSIVLLLQCDIQK Predict reactive species
Full Name	visinin-like 1
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	20 kDa
GenBank Accession Number	BC022012
Gene Symbol	VSNL1
Gene ID (NCBI)	7447
RRID	AB_2882433
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P62760
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Visinin-like protein 1 (VILIP1) is also named VLP-1, hippocalcin-like protein 3 (HLP3), VSNL1 and VISL1. The neuronal calcium sensor protein VILIP1 forms a complex with neurotransmitter-gated P2X2 receptors, functions as a channel regulator, and likely binds more tightly to P2X2 receptors in an activation- and calcium-dependent manner (PMID: 18922787). VILIP1 is widely expressed and involved in physiological and disease processes throughout the body, including tumor formation (PMID: 17311005, PMID: 18301774). VILIP1 is localized primarily in the rat hippocampus, where it controls neuronal excitability important for learning and memory (PMID: 18440708, PMID: 15336574). In particular, VILIP1 binds to the α-subunit of the α4β2 nicotinic acetylcholine receptor (nAChR), which promotes its surface expression and trafficking in oocytes and hippocampal neurons (PMID: 1220248, PMID: 19063970).

Protocols

Product Specific Protocols
IHC protocol for VILIP1 antibody 67134-1-Ig	Download protocol
WB protocol for VILIP1 antibody 67134-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The VILIP1 Monoclonal antibody (2C6C3), catalog number 67134-1-Ig, has a total of 1 citation. This citation appears in the journal Biomedicines (2023, Impact Factor 4.5). The associated research field is cancer bioinformatics, specifically the analysis of featured genes linked to cholangiocarcinoma progression, with the antibody applied in IHC on human samples.