VSNL1 Monoclonal antibody

VSNL1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2C6C3

Cat no : 67134-1-Ig

Synonyms

Hippocalcin like protein 3, HLP3, HPCAL3, HUVISL1, VILIP, VILIP 1, visinin like 1, Visinin like protein 1, VISL1, VLP 1, VSNL1

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Validation Data Gallery

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  WB analysis of human brain using 67134-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 67134-1-Ig (VSNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67134-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67134-1-Ig (VSNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67134-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67134-1-Ig (VSNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67134-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67134-1-Ig (VSNL1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67134-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67134-1-Ig (VSNL1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

67134-1-Ig targets VSNL1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VSNL1 fusion protein Ag4923
• Full Name: visinin-like 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC022012
• Gene Symbol: VSNL1
• Gene ID (NCBI): 7447
• RRID: AB_2882433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Biomedicines; Characteristic Analysis of Featured Genes Associated with Cholangiocarcinoma Progression; Authors - Qigu Yao