VTI1B Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87150-1-RR (same clone as 87150-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 87150-1-RR (VTI1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87150-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 87150-1-RR (same clone as 87150-1-PBS)

  IP result of anti-VTI1B (IP:87150-1-RR, 4ug; Detection:87150-1-RR 1:800) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 87150-1-PBS in a different storage buffer formulation.

VTI1B Uni-rAb^® Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 87150-1-PBS

Clone No.252349D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Vesicle transport through interaction with t-SNAREs homolog 1B, Vesicle transport v-SNARE protein Vti1-like 1, VTI1, VTI1 LIKE, Vti1 rp1

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Product Information

87150-1-PBS targets VTI1B in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5906

  Product name: Recombinant human VTI1B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 13-232 aa of BC003142

  Sequence: KLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLLSIIILLELAILGGLVYYKFFRSH

• Full Name: vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC003142
• Gene Symbol: VTI1B
• Gene ID (NCBI): 10490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UEU0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp, which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8, and VAMP8, which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7, and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).