Back

Vimentin Antibody 12 Publications

Mouse Monoclonal| Catalog number: 60330-1-Ig |CloneNo.: 3H9D1

Featured Product KD/KO validated

  • Print page
  • Download PDF

Be the first to review this product

-_-

Freight/Packing

Con: 120 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Related Primary Antibodies
Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

Vimentin Antibody IHC human colon tissue 60330-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:102400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Vimentin Antibody IHC human liver cancer tissue 60330-1-Ig

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Vimentin Antibody IHC human breast cancer tissue 60330-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Vimentin Antibody IHC human breast cancer tissue 60330-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Vimentin Antibody WB Jurkat cells 60330-1-Ig

WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

Vimentin Antibody IHC human tonsillitis tissue 60330-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:4000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Vimentin Antibody WB Raji cells 60330-1-Ig

Raji cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig( Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

Vimentin Antibody IF HepG2 cells 60330-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 60330-1-Ig (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Vimentin Antibody FC Jurkat cells 60330-1-Ig

1X10^6 Jurkat cells were stained with 0.20ug Vimentin antibody (60330-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH

Species specificity:
human, mouse, rat
Positive WB detected in:
Raji cells, Jurkat cells, A549 cells, HeLa cells, ROS1728 cells, NIH/3T3 cells
Positive IHC detected in:
human tonsillitis tissue, human breast cancer tissue, human pancreas tissue, human colon tissue, human renal cell carcinoma tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, HeLa cells
Positive FC detected in:
Jurkat cells
Recommended dilution:
WB : 1:10000-1:50000
IHC : 1:2000-1:10000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Vimentin fusion protein Ag0489
Full name:
vimentin
Calculated molecular weight:
466aa,54 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
BC000163
Gene ID (NCBI):
7431
Gene symbol
VIM
Synonyms
VIM, Vimentin

Background

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.


Back
to top