A549 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig( Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig( Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig( Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig( Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

Raji cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig( Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

ROS1728 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig( Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig( Vimentin Antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig( Vimentin Antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunofluorescent analysis of HeLa cells using 60330-1-Ig(Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

1X10^6 Jurkat cells were stained with 0.20ug Vimentin antibody (60330-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH