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Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat and More (1)







Cat no : 60330-1-Ig


VIM, Vimentin

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, ROS1728 cells, Jurkat cells, A549 cells, U2OS cells, U-251 cells
Positive IHC detected inhuman appendicitis tissue, human renal cell carcinoma tissue, human endometrial cancer tissue, human ovary tumor tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue, human tonsillitis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, Retinal organoids, HepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60330-1-Ig targets Vimentin in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489
Full Name vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.


Product Specific Protocols
WB protocol for Vimentin antibody 60330-1-IgDownload protocol
IHC protocol for Vimentin antibody 60330-1-IgDownload protocol
IF protocol for Vimentin antibody 60330-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

  • Applications: Immunofluorescence
  • Cell Tissue Type: Retinal organoids

Aarushi (Verified Customer) (04-15-2023)

Primary antibody worked well after methanol treatment in a dilution of 1:200.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Tanycyte monolayer cell cultures (mouse)
Vimentin Antibody Immunofluorescence validation (1:200 dilution) in Tanycyte monolayer cell cultures (mouse) (Cat no:60330-1-Ig)

Saba (Verified Customer) (03-03-2022)

The antibody worked perfectly!! The band was sharp and no unspecefic bands were seen.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF10A cells