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Vimentin Antibody 5 Publications

Mouse Monoclonal| Catalog number: 60330-1-Ig |CloneNo.: 3H9D1

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Con: 120 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Vimentin Antibody WB Jurkat cells 60330-1-Ig

WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

Vimentin Antibody IHC human tonsillitis tissue 60330-1-Ig

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:4000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Vimentin Antibody IF HepG2 cells 60330-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 60330-1-Ig (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Vimentin Antibody FC Jurkat cells 60330-1-Ig

1X10^6 Jurkat cells were stained with 0.20ug Vimentin antibody (60330-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, A549 cells, HeLa cells, NIH/3T3 cells, Raji cells, ROS1728 cells
Positive IHC detected in:
human tonsillitis tissue, human colon tissue, human breast cancer tissue, human renal cell carcinoma tissue
Positive IF detected in:
HepG2 cells, HeLa cells
Positive FC detected in:
Jurkat cells
Recommended dilution:
WB : 1:1000-1:8000
IHC : 1:2000-1:4000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Vimentin fusion protein Ag0489
Full name:
vimentin
Calculated molecular weight:
466aa,54 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
BC000163
Gene ID (NCBI):
7431
Gene symbol
VIM
Synonyms
VIM, Vimentin

Background

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.


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