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Vimentin Monoclonal antibody
Vimentin Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
CloneNo.
3H9D1
Cat no : 60330-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Jurkat cells, A549 cells, HeLa cells, ROS1728 cells, NIH/3T3 cells |
| Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue, human colon tissue, human endometrial cancer tissue, human liver cancer tissue, human ovary tumor tissue, human pancreas tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, HeLa cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 43 publications below |
| IHC | See 6 publications below |
| IF | See 21 publications below |
| IP | See 1 publications below |
Product Information
60330-1-Ig targets Vimentin in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Vimentin fusion protein Ag0489 |
| Full Name | vimentin |
| Calculated molecular weight | 466 aa, 54 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC000163 |
| Gene symbol | VIM |
| Gene ID (NCBI) | 7431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Vimentin antibody 60330-1-Ig | Download protocol |
| IHC protocol for Vimentin antibody 60330-1-Ig | Download protocol |
| IF protocol for Vimentin antibody 60330-1-Ig | Download protocol |
| FC protocol for Vimentin antibody 60330-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression. | ||
Cancer Cell Int SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition. | ||
Neurochem Res Resistin-Inhibited Neural Stem Cell-Derived Astrocyte Differentiation Contributes to Permeability Destruction of the Blood-Brain Barrier. | ||
Hum Reprod Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis. | ||
Biomed Res Int Electrical Stimulation Activates Fibroblasts through the Elevation of Intracellular Free Ca2+: Potential Mechanism of Pelvic Electrical Stimulation Therapy. | ||
J Exp Clin Cancer Res RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer. |
