Biotin-conjugated Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

CloneNo.

3H9D1

Cat no : Biotin-60330

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

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Validation Data Gallery

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  IHC staining of human tonsillitis using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using Biotin-60330

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-60330 (Vimentin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-60330 targets Vimentin in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Vimentin fusion protein Ag0489
• Full Name: vimentin
• Calculated Molecular Weight: 466 aa, 54 kDa
• GenBank Accession Number: BC000163
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• RRID: AB_2883066
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. And this antibody is conjugated with biotin.