CoraLite®488-conjugated Vimentin Monoclonal antibody

Vimentin Monoclonal Antibody for FC, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3H9D1

Cat no : CL488-60330

Synonyms

VIM, vimentin



Tested Applications

Positive IF detected inhuman tonsillitis tissue, HepG2 cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 60330-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60330 targets Vimentin in IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated Vimentin antibody CL488-60330Download protocol
FC protocol for CL488-conjugated Vimentin antibody CL488-60330Download protocol
Standard Protocols
Click here to view our Standard Protocols