CoraLite®555-conjugated Vimentin Monoclonal antibody

• Featured Product
• KD/KO Validated

Vimentin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3H9D1

Cat no : CL555-60330

Synonyms

FLJ36605, VIM, vimentin

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Validation Data Gallery

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  IF Staining of HepG2 using CL555-60330

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 Vimentin antibody (CL555-60330, Clone: 3H9D1 ) at dilution of 1:400.

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  IF Staining of HeLa using CL555-60330

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®555 Vimentin antibody (CL555-60330, Clone: 3H9D1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-60330 targets Vimentin in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Vimentin fusion protein Ag0489
• Full Name: vimentin
• Calculated Molecular Weight: 466 aa, 54 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC000163
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• RRID: AB_2919663
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.