WDFY2 Polyclonal antibody

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  WB analysis using 17197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17197-1-AP (WDFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WDFY2 Polyclonal Antibody for WB, ELISA

Cat No. 17197-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ZFYVE22, WDF2, WD40- and FYVE domain-containing protein 2, WD repeat and FYVE domain-containing protein 2, Propeller-FYVE protein

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Tested Applications

• Positive WB detected in: L-929 cells, 3T3-L1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17197-1-AP targets WDFY2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDFY2 fusion protein Ag10415
• Full Name: WD repeat and FYVE domain containing 2
• Calculated Molecular Weight: 400 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC014004
• Gene Symbol: WDFY2
• Gene ID (NCBI): 115825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96P53
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDFY2, also named Prof, is a protein that contains WD repeats and an FYVE domain. WDFY2 regulates the membrane-bound matrix metalloproteinase MT1-MMP (MMP14) efflux by controlling endosome sorting. WDFY2 may act as a tumor suppressor by limiting VAMP3-dependent recycling to inhibit matrix metalloproteinase secretion and cell invasion. (PMID: 31253801)