WDR37 Polyclonal antibody

WDR37 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20916-1-AP

Synonyms

KIAA0982, RP11 529L18.2, WD repeat domain 37, WDR37

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Validation Data Gallery

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  WB analysis using 20916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20916-1-AP (WDR37 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20916-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20916-1-AP (WDR37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

20916-1-AP targets WDR37 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR37 fusion protein Ag15112
• Full Name: WD repeat domain 37
• Calculated Molecular Weight: 494 aa, 55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC018044
• Gene Symbol: WDR37
• Gene ID (NCBI): 22884
• RRID: AB_10695501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of WDR37 remains largely unknown. Catalog#20916-1-AP is a rabbit polyclonal antibody raised against the full-length of human WDR37. The MW of this protein is 55 kDa, and this antibody specially recognises the 55 kDa protein.

Publications

Species	Application	Title
mouse	IHC	ACS Nano; Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.; Authors - Yakun Wu
human	IHC	Front Immunol; An E3 ubiquitin-proteasome gene signature for predicting prognosis in patients with pancreatic cancer; Authors - Taoyuan Yin