Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse cerebellum tissue, rat kidney tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IP | See 1 publications below |
Product Information
29997-1-AP targets WDR73 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WDR73 fusion protein Ag31818 Predict reactive species |
| Full Name | WD repeat domain 73 |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC063392 |
| Gene Symbol | WDR73 |
| Gene ID (NCBI) | 84942 |
| RRID | AB_3086206 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P4I2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WDR73 contains multiple WD40 repeat domains that function as scaffolds for the assembly of protein complexes. WDR73 was present in the brain and kidney and was located diffusely in the cytoplasm during interphase but relocalized to spindle poles and astral microtubules during mitosis. WDR73 interacts with microtubules to regulate cell cycle progression, proliferation, and survival in the brain and kidney(PMID: 26070982). Reduced expression of WDR73 results in abnormalities in the size and morphology of the nucleus. Mutations in WDR73 have been associated with Galloway-Mowat syndrome, which is a rare autosomal recessive disorder that affects both the central nervous system and the kidney (PMID: 25466283).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR73 antibody 29997-1-AP | Download protocol |
| IHC protocol for WDR73 antibody 29997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
