WDR73 Polyclonal antibody

WDR73 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29997-1-AP

Synonyms

FLJ14888, HSPC264, WD repeat domain 73, WDR73

---

Validation Data Gallery

• 

  WB analysis using 29997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29997-1-AP (WDR73 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse cerebellum tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29997-1-AP targets WDR73 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR73 fusion protein Ag31818
• Full Name: WD repeat domain 73
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC063392
• Gene Symbol: WDR73
• Gene ID (NCBI): 84942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR73 contains multiple WD40 repeat domains that function as scaffolds for the assembly of protein complexes. WDR73 was present in the brain and kidney and was located diffusely in the cytoplasm during interphase but relocalized to spindle poles and astral microtubules during mitosis. WDR73 interacts with microtubules to regulate cell cycle progression, proliferation, and survival in the brain and kidney(PMID: 26070982). Reduced expression of WDR73 results in abnormalities in the size and morphology of the nucleus. Mutations in WDR73 have been associated with Galloway-Mowat syndrome, which is a rare autosomal recessive disorder that affects both the central nervous system and the kidney (PMID: 25466283).