WDR74 Polyclonal antibody, PBS Only

• 

  WB analysis using 20631-1-AP (same clone as 20631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20631-1-AP (same clone as 20631-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 20631-1-AP (same clone as 20631-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 20631-1-AP (same clone as 20631-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20631-1-AP (WDR74 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20631-1-PBS in a different storage buffer formulation.

WDR74 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 20631-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

NOP seven associated protein 1, NOP seven-associated protein 1, NSA1, WD repeat domain 74, WD repeat-containing protein 74

---

Product Information

20631-1-PBS targets WDR74 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR74 fusion protein Ag14617
• Full Name: WD repeat domain 74
• Calculated Molecular Weight: 385 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC006351
• Gene Symbol: WDR74
• Gene ID (NCBI): 54663
• RRID: AB_2878713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6RFH5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

WDR74, also known as WD repeat domain 74, is a protein-coding gene that plays important roles in various biological processes. WDR74 is involved in rRNA processing and ribosomal large subunit biogenesis. It is a 60S ribosome assembly factor, which is irreplaceable in ribosome biogenesis and is closely related to protein synthesis. WDR74 can induce nuclear β-catenin accumulation and activate Wnt-responsive genes, thus promoting cell proliferation and metastasis in various cancers.