WDR77 Polyclonal antibody

WDR77 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10115-1-AP

Synonyms

Androgen receptor cofactor p44, HKMT1069, MEP 50, MEP50, Methylosome protein 50, Nbla10071, p44, p44/Mep50, RP11 552M11.3, WD repeat domain 77, WDR77

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Validation Data Gallery

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  WB analysis using 10115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10115-1-AP (WDR77 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Product Information

10115-1-AP targets WDR77 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR77 fusion protein Ag0147
• Full Name: WD repeat domain 77
• Calculated molecular weight: 50 kDa
• Observed molecular weight: 40 kDa
• GenBank accession number: BC001679
• Gene symbol: WDR77
• Gene ID (NCBI): 79084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR77, other named p44, MEP-50, is a component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. Normally, WDR77 locates in the nucleus, such as in prostate epithelial cells. However, it localized to the cytoplasm in prostatic intraepithelial neoplasia and prostate cancer cells. Nuclear WDR77 mediates the growth inhibition and differentiation. WDR may involve in the biogenesis of small nuclear ribonucleoprotein particles.