WDR77 Polyclonal antibody

WDR77 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10115-1-AP

Synonyms

WDR 77, WD45, WD repeat-containing protein 77, Nbla10071, Methylosome protein WDR77

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Validation Data Gallery

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  WB analysis using 10115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10115-1-AP (WDR77 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 10115-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10115-1-AP (WDR77 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10115-1-AP targets WDR77 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR77 fusion protein Ag0147
• Full Name: WD repeat domain 77
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC001679
• Gene Symbol: WDR77
• Gene ID (NCBI): 79084
• RRID: AB_2215725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR77, other named p44, MEP-50, is a component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. Normally, WDR77 locates in the nucleus, such as in prostate epithelial cells. However, it localized to the cytoplasm in prostatic intraepithelial neoplasia and prostate cancer cells. Nuclear WDR77 mediates the growth inhibition and differentiation. WDR may involve in the biogenesis of small nuclear ribonucleoprotein particles.

Publications

Species	Application	Title
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun