Validation Data Gallery
|Positive IHC detected in||rat brain tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
26995-1-AP targets WFS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WFS1 fusion protein Ag25724|
|Full Name||Wolfram syndrome 1 (wolframin)|
|Calculated molecular weight||890 aa, 100 kDa|
|GenBank accession number||BC030130|
|Gene ID (NCBI)||7466|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.
Sci Transl Med
Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice.
Wolframin is a novel regulator of tau pathology and neurodegeneration.
WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells.
The fasciola cinereum subregion of the hippocampus is important for the acquisition of visual contextual memory.
Acta Neuropathol Commun
Wolfram syndrome 1b mutation suppresses Mauthner-cell axon regeneration via ER stress signal pathway
J Med Genet
WFS1 protein expression correlates with clinical progression of optic atrophy in patients with Wolfram syndrome.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jordan (Verified Customer) (01-25-2021)
Used free floating staining 16 hours in primary and 6 hours in secondary (Alexa 488+) at 4 degrees C. Antibody does a perfect job of labelling MEC layer 2 pyramidal neurons, alongside Parasubiculum neurons, some neurons in the subiculum and the majority of CA1 pyramidal neurons as widely reported in the literature