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  • KD/KO Validated

WFS1 Polyclonal antibody

WFS1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26995-1-AP

Synonyms

WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN



Tested Applications

Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26995-1-AP targets WFS1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WFS1 fusion protein Ag25724
Full Name Wolfram syndrome 1 (wolframin)
Calculated Molecular Weight 890 aa, 100 kDa
GenBank Accession NumberBC030130
Gene Symbol WFS1
Gene ID (NCBI) 7466
RRIDAB_2880717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

Protocols

Product Specific Protocols
IHC protocol for WFS1 antibody 26995-1-APDownload protocol
IF protocol for WFS1 antibody 26995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Transl Med

Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice.

Authors - Jean-Christophe Delpech
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
human,ratWB,IF

Nat Commun

WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells.

Authors - Linlin Wang
  • KO Validated
ratIHC

Prog Neurobiol

The fasciola cinereum subregion of the hippocampus is important for the acquisition of visual contextual memory.

Authors - Seong-Beom Park
mouseIF

Cell Rep

Acetylcholine synergizes with netrin-1 to drive persistent firing in the entorhinal cortex

Authors - Stephen D Glasgow
mouseIHC

Elife

The differentiation and integration of the hippocampal dorsoventral axis are controlled by two nuclear receptor genes

Authors - Xiong Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jordan (Verified Customer) (01-25-2021)

Used free floating staining 16 hours in primary and 6 hours in secondary (Alexa 488+) at 4 degrees C. Antibody does a perfect job of labelling MEC layer 2 pyramidal neurons, alongside Parasubiculum neurons, some neurons in the subiculum and the majority of CA1 pyramidal neurons as widely reported in the literature

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse Brain/Pyramidal Neurons