WNT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

WNT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 27214-1-AP

Synonyms

Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

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Validation Data Gallery

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  WB analysis using 27214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27214-1-AP (WNT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

The immunogen of 27214-1-AP is WNT2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT2 fusion protein Ag25845
• Full Name: wingless-type MMTV integration site family member 2
• Calculated molecular weight: 40 kDa
• Observed molecular weight: 40 kDa
• GenBank accession number: BC029854
• Gene symbol: WNT2
• Gene ID (NCBI): 7472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.; Authors - Xi Dong
mouse	WB	FASEB J; Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2.; Authors - Yunzhen Ye
human	WB	Phytother Res; Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway.; Authors - Mingming Zhu
mouse	WB	Brain Res Bull; Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.; Authors - Qian Wu
human	WB	Cancer Med; Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling.; Authors - Xia Liu ; KD Validated
human	WB	Aging (Albany NY); LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.; Authors - Liting Jin