|Positive WB detected in||mouse brain tissue, rat brain tissue, MCF-7 cells|
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
27214-1-AP targets WNT2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WNT2 fusion protein Ag25845|
|Full Name||wingless-type MMTV integration site family member 2|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||40 kDa, 34 kDa|
|GenBank accession number||BC029854|
|Gene ID (NCBI)||7472|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.
Clin Transl Med
Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.
Aging (Albany NY)
LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.
Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2.
Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway.
Brain Res Bull
Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.
Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling.