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  • KD/KO Validated

WNT2 Polyclonal antibody

WNT2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27214-1-AP

Synonyms

Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, Jurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27214-1-AP is WNT2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT2 fusion protein Ag25845
Full Name wingless-type MMTV integration site family member 2
Calculated molecular weight 40 kDa
GenBank accession numberBC029854
Gene symbol WNT2
Gene ID (NCBI) 7472
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WNT2 antibody 27214-1-APDownload protocol
IHC protocol for WNT2 antibody 27214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Genomics

Comparative study on seasonal hair follicle cycling by analysis of the transcriptomes from cashmere and milk goats.

Authors - Yanjun Zhang
humanWB

Cancer Med

Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling.

Authors - Xia Liu
  • KD Validated
humanWB

Phytother Res

Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway.

Authors - Mingming Zhu
mouseWB

FASEB J

Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2.

Authors - Yunzhen Ye
mouseWB

Clin Transl Med

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Authors - Xi Dong