• Featured Product
  • KD/KO Validated

WNT2 Polyclonal antibody

WNT2 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 27214-1-AP


Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, MCF-7 cells
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27214-1-AP targets WNT2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT2 fusion protein Ag25845
Full Name wingless-type MMTV integration site family member 2
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40 kDa, 34 kDa
GenBank Accession NumberBC029854
Gene Symbol WNT2
Gene ID (NCBI) 7472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.


Product Specific Protocols
WB protocol for WNT2 antibody 27214-1-APDownload protocol
IHC protocol for WNT2 antibody 27214-1-APDownload protocol
IF protocol for WNT2 antibody 27214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Clin Transl Med

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Authors - Xi Dong

Aging (Albany NY)

LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.

Authors - Liting Jin


Bisphenol A exposure alters placentation and causes preeclampsia-like features in pregnant mice involved in reprogramming of DNA methylation of WNT2.

Authors - Yunzhen Ye

Phytother Res

Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β-catenin pathway.

Authors - Mingming Zhu

Brain Res Bull

Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.

Authors - Qian Wu

Cancer Med

Mycoplasma hyorhinis infection promotes gastric cancer cell motility via β-catenin signaling.

Authors - Xia Liu
  • KD Validated