|Positive WB detected in||Jurkat cells, HeLa cells, HepG2 cells, L02 cells, MOLT-4 cells|
|Positive IP detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
10200-1-AP targets WTAP in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WTAP fusion protein Ag0259|
|Full Name||Wilms tumor 1 associated protein|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC000383|
|Gene ID (NCBI)||9589|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.
m6A RNA methylation regulates the fate of endogenous retroviruses.
METTL3 regulates heterochromatin in mouse embryonic stem cells.
Nat Cell Biol
The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.
Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.
Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.
Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)