WTAP Polyclonal antibody

• Featured Product
• KD/KO Validated

WTAP Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 10200-1-AP

Synonyms

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

---

Validation Data Gallery

• 

  WB analysis of HeLa using 10200-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10200-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat cells using 10200-1-AP

  WB result of WTAP specific antibody (10200-1-AP, 1:6000) with si-control and si-WTAP transfected Jurkat cells.

• 

  WB analysis using 10200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10200-1-AP

  IP Result of anti-WTAP (IP:10200-1-AP, 3ug; Detection:10200-1-AP 1:500) with HEK-293 cells lysate 2500ug.

• 

  IP experiment of Jurkat using 10200-1-AP

  IP result of anti-WTAP(IP:10200-1-AP, 4ug; Detection:10200-1-AP 1:8000) with Jurkat cells lysate 2280 ug.

---

Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, MOLT-4 cells, L02 cells, K-562 cells
• Positive IP detected in: Jurkat cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 11 publications below
• WB: See 37 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• RIP: See 3 publications below

Product Information

10200-1-AP targets WTAP in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WTAP fusion protein Ag0259
• Full Name: Wilms tumor 1 associated protein
• Calculated molecular weight: 45 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC000383
• Gene symbol: WTAP
• Gene ID (NCBI): 9589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.

Publications

Species	Application	Title
mouse	WB	Nature; m6A RNA methylation regulates the fate of endogenous retroviruses.; Authors - Tomasz Chelmicki ; KO Validated
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu ; KD Validated
mouse	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human	WB	Mol Cell; Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.; Authors - Wenqi Xu ; KD Validated
mouse	WB,IF	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li

Related Products

WT1 Polyclonal antibody

12609-1-AP

GPR132 Polyclonal antibody

17026-1-AP

POU4F2 Polyclonal antibody

55042-1-AP

CENPA Polyclonal antibody

26754-1-AP

Cyclin A2 Polyclonal antibody

18202-1-AP