• Featured Product
  • KD/KO Validated

WTAP Polyclonal antibody

WTAP Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 10200-1-AP

Synonyms

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, MCF-7 cells, HepG2 cells, L02 cells, MOLT-4 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

10200-1-AP targets WTAP in WB, RIP, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WTAP fusion protein Ag0259
Full Name Wilms tumor 1 associated protein
Calculated molecular weight 45 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000383
Gene symbol WTAP
Gene ID (NCBI) 9589
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for WTAP antibody 10200-1-APDownload protocol
IP protocol for WTAP antibody 10200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA Biol

Reprogramming of m6A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia.

Authors - Yan-Jie Wang
mouseIF

Cell Rep

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Authors - Benoit Moindrot
humanIF

Mol Cancer

WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1.

Authors - Yunhao Chen
  • KD Validated
mouseWB,IF

Mol Cell

Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Authors - Jing Wen
mouseWB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
mouseWB

Nature

m6A RNA methylation regulates the fate of endogenous retroviruses.

Authors - Tomasz Chelmicki
  • KO Validated