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WTAP Monoclonal antibody
WTAP Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, mouse, rat and More (1)
WB, IP, IHC, IF, FC, ELISA
Cat no : 60188-1-Ig
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 5 publications below|
|WB||See 42 publications below|
|IHC||See 11 publications below|
|IF||See 5 publications below|
|IP||See 3 publications below|
|FC||See 1 publications below|
60188-1-Ig targets WTAP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, drosophila|
|Host / Isotype||Mouse / IgG1|
|Immunogen||WTAP fusion protein Ag0259|
|Full Name||Wilms tumor 1 associated protein|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC000383|
|Gene ID (NCBI)||9589|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.
|Product Specific Protocols|
|WB protocol for WTAP antibody 60188-1-Ig||Download protocol|
|IHC protocol for WTAP antibody 60188-1-Ig||Download protocol|
|IF protocol for WTAP antibody 60188-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.
Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.
Synaptic N6-methyladenosine (m6A) epitranscriptome reveals functional partitioning of localized transcripts.
Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.
mTORC1 promotes cell growth via m6A-dependent mRNA degradation.
The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Elaine (Verified Customer) (01-20-2021)
Antibody works fine in Western blot on MEF cells
Daniel (Verified Customer) (06-25-2019)
RCC4 grown in normoxic (left lane) and hypoxic (right lane) conditions.