• Featured Product
  • KD/KO Validated

WTAP Monoclonal antibody

WTAP Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat and More (1)







Cat no : 60188-1-Ig


Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60188-1-Ig targets WTAP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, drosophila
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WTAP fusion protein Ag0259
Full Name Wilms tumor 1 associated protein
Calculated molecular weight 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000383
Gene symbol WTAP
Gene ID (NCBI) 9589
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.


Product Specific Protocols
WB protocol for WTAP antibody 60188-1-IgDownload protocol
IHC protocol for WTAP antibody 60188-1-IgDownload protocol
IF protocol for WTAP antibody 60188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Authors - Shay Geula

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
mouseIHC, IF

Nat Neurosci

Synaptic N6-methyladenosine (m6A) epitranscriptome reveals functional partitioning of localized transcripts.

Authors - Daria Merkurjev

Mol Cell

Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

Authors - Nandan S Gokhale

Mol Cell

mTORC1 promotes cell growth via m6A-dependent mRNA degradation.

Authors - Sungyun Cho
  • KO Validated

Mol Cell

The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.

Authors - Shuibin Lin
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Elaine (Verified Customer) (01-20-2021)

Antibody works fine in Western blot on MEF cells

  • Applications: Western Blot

Daniel (Verified Customer) (06-25-2019)

RCC4 grown in normoxic (left lane) and hypoxic (right lane) conditions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: RCC4 human cells
WTAP Antibody Western Blot, validation (1:2000 dilution) in RCC4 human cells (Cat no:60188-1-Ig)