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WTAP Monoclonal antibody
WTAP Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 60188-1-Ig
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, LNCaP cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60188-1-Ig targets WTAP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, drosophila|
|Host / Isotype||Mouse / IgG1|
|Immunogen||WTAP fusion protein Ag0259|
|Full Name||Wilms tumor 1 associated protein|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC000383|
|Gene ID (NCBI)||9589|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.
The function of Wtap in N6-adenosine methylation of mRNAs controls T cell receptor signaling and survival of T cells.
Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d.
Med Sci Monit
Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.
Alteration of N6-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.
Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m 6 A Methylation
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Elaine (Verified Customer) (01-20-2021)
Antibody works fine in Western blot on MEF cells
Daniel (Verified Customer) (06-25-2019)
RCC4 grown in normoxic (left lane) and hypoxic (right lane) conditions.