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  • KD/KO Validated

WTAP Monoclonal antibody

WTAP Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

4A10G9

Cat no : 60188-1-Ig

Synonyms

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, mouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60188-1-Ig targets WTAP in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity drosophila, human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WTAP fusion protein Ag0259
Full Name Wilms tumor 1 associated protein
Calculated molecular weight 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000383
Gene symbol WTAP
Gene ID (NCBI) 9589
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for WTAP antibody 60188-1-IgDownload protocol
IHC protocol for WTAP antibody 60188-1-IgDownload protocol
IF protocol for WTAP antibody 60188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
drosophilaWB

Genes Dev

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d.

Authors - Philip Knuckles
humanWB

Genes Dev

PMID: 30463905

Authors - Rosa M Rubio
humanIHC

Med Sci Monit

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Authors - Yingying Zhang
mouseWB

Epigenomics

Alteration of N6-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.

Authors - Xueyan Li
humanWB,IP

Mol Cell

Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m 6 A Methylation

Authors - Hui-Lung Sun
humanWB

Cell Death Dis

Suppression of m6A mRNA modification by DNA hypermethylated ALKBH5 aggravates the oncological behavior of KRAS mutation/LKB1 loss lung cancer.

Authors - Donghong Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elaine (Verified Customer) (01-20-2021)

Antibody works fine in Western blot on MEF cells

  • Applications: Western Blot
FH

Daniel (Verified Customer) (06-25-2019)

RCC4 grown in normoxic (left lane) and hypoxic (right lane) conditions.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: RCC4 human cells
WTAP Antibody Western Blot, validation (1:2000 dilution) in RCC4 human cells (Cat no:60188-1-Ig)