Validation Data Gallery
|Positive WB detected in||HeLa cells, MCF-7 cells, HepG2 cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human liver cancer tissue, human ovary tumor tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||NIH3T3 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13584-1-AP targets YAP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, chicken, monkey, pig, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||YAP1 fusion protein Ag4510|
|Full Name||Yes-associated protein 1, 65kDa|
|Calculated molecular weight||504 aa, 54 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC038235|
|Gene ID (NCBI)||10413|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).This antibody is a rabbit polyclonal antibody. The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65kDa. (PMID: 26695440)
|Product Specific Protocols|
|WB protocol for YAP1 antibody 13584-1-AP||Download protocol|
|IHC protocol for YAP1 antibody 13584-1-AP||Download protocol|
|IF protocol for YAP1 antibody 13584-1-AP||Download protocol|
|IP protocol for YAP1 antibody 13584-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling.
The Hippo Pathway Regulates Caveolae Expression and Mediates Flow Response via Caveolae.
Circular RNA Vav3 sponges gga-miR-375 to promote epithelial-mesenchymal transition.
Onco Targets Ther
Downregulation of CENPK suppresses hepatocellular carcinoma malignant progression through regulating YAP1.
F-actin dynamics regulates mammalian organ growth and cell fate maintenance.
Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
María (Verified Customer) (02-08-2022)
Works for WB (1:1000).
Arianna (Verified Customer) (03-01-2019)
Genetically validated on YAP-null liver sections.
Joshua (Verified Customer) (12-20-2018)
Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with DAPI and phalloidin. Stain was mix of nuclear, cytosolic, and junctional.
Joshua (Verified Customer) (12-20-2018)
Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with phalliodin and DAPI. Staining is mix of nuclear, junctional, and cytosolic.
Juan Pablo (Verified Customer) (11-29-2018)
CGN treated with Shh (1ug/ml) for 48hs to induced proliferation, I see a nice induction of YAP1I get a nice clean band
kk (Verified Customer) (11-21-2018)