YES1-Specific Polyclonal antibody

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  WB analysis using 20243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20243-1-AP (YES1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20243-1-AP

  WB result of YES1-Specific antibody (20243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1-Specific transfected HepG2 cells.

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  IP experiment of A549 using 20243-1-AP

  IP result of anti-YES1-Specific (IP:20243-1-AP, 4ug; Detection:20243-1-AP 1:500) with A549 cells lysate 1400 ug.

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  IHC staining of human lung cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

YES1-Specific Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20243-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

YES1, c yes, EC:2.7.10.2, HsT441, P61 YES

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, A549 cells, DU 145 cells, HEK-293 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

20243-1-AP targets YES1-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: NM_005433
• Gene Symbol: YES1
• Gene ID (NCBI): 7525
• RRID: AB_10697656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07947
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Am J Respir Crit Care Med; YES1 Drives Lung Cancer Growth and Progression and Predicts Sensitivity to Dasatinib.; Authors - Irati Garmendia
mouse,human	WB	Sci Adv; A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.; Authors - Jackson A Roberts
human	WB,IHC	J Immunother Cancer; SRC family kinase (SFK) inhibitor dasatinib improves the antitumor activity of anti-PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferation.; Authors - Esther Redin ; KO Validated
human	WB	Cell Death Dis; Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p.; Authors - Yujing Sun
human	WB	J Cell Biochem; miR-17-5p up-regulates YES1 to modulate the cell cycle progression and apoptosis in ovarian cancer cell lines.; Authors - Lan Li ; KD Validated
human	WB	iScience; DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer; Authors - Qingwen Huang