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WB analysis using 83970-1-RR (same clone as 83970-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 83970-1-RR (same clone as 83970-1-PBS)
WB result of YTHDC2 antibody (83970-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP00893-3
The mean YTHDC2 concentration was determined to be 4.89 ng/mL in HEK-293T cell extract based on a 1.4 mg/mL extract load.
Sandwich ELISA standard curve of MP00893-3
Sandwich ELISA standard curve of MP00893-3, Human YTHDC2 Recombinant Matched Antibody Pair - PBS only. 83970-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26690. 83970-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL
IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 83970-1-RR (same clone as 83970-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83970-1-RR
Biolayer interferometry (BLl) kinetic assays of 83970-1-RR against Human YTHDC2 were performed. The affinity constant is 0.378 nM.

Featured Product
KD/KO Validated

YTHDC2 Recombinant monoclonal antibody, PBS Only (Detector)

YTHDC2 Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

Cat No. 83970-1-PBS

Clone No.241123A9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA

Synonyms

241123A9, 3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83970-1-PBS targets YTHDC2 as part of a matched antibody pair:

MP00893-3: 83970-3-PBS capture and 83970-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag26690 Product name: Recombinant human YTHDC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1131-1430 aa of BC137285 Sequence: HSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSSADTEFSDECTTAERVLMKSPSPALHPPQKYKDRGILHPKRGTEDRSDQSSLKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD Predict reactive species
Full Name	YTH domain containing 2
Observed Molecular Weight	160 kDa
GenBank Accession Number	BC137285
Gene Symbol	YTHDC2
Gene ID (NCBI)	64848
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9H6S0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.