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- KD/KO Validated
YTHDF1 Monoclonal antibody
YTHDF1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse and More (2)
Applications
WB, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
3A2H12
Cat no : 66745-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 2 publications below |
IF | See 1 publications below |
CoIP | See 1 publications below |
Product Information
66745-1-Ig targets YTHDF1 in WB, IF, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | YTHDF1 fusion protein Ag11633 |
Full Name | YTH domain family, member 1 |
Calculated molecular weight | 559 aa, 61 kDa |
Observed molecular weight | 60 kDa |
GenBank accession number | BC050284 |
Gene symbol | YTHDF1 |
Gene ID (NCBI) | 54915 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for YTHDF1 antibody 66745-1-Ig | Download protocol |
IHC protocol for YTHDF1 antibody 66745-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.
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EMBO Rep N(6)-methyladenosine-binding protein YTHDF1 suppresses EBV replication and promotes EBV RNA decay.
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Apoptosis NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer
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