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  • KD/KO Validated

YWHAH Polyclonal antibody

YWHAH Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 15222-1-AP

Synonyms

14 3 3 protein eta, 14-3-3, 14-3-3 eta, Protein AS1, YWHA1, YWHAH



Tested Applications

Positive WB detected inhuman brain tissue, A431 cells, mouse brain tissue, NIH/3T3 cells, Raji cells
Positive IP detected inNIH/3T3 cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15222-1-AP targets YWHAH in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YWHAH fusion protein Ag7435
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC003047
Gene symbol YWHAH
Gene ID (NCBI) 7533
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.

Protocols

Product Specific Protocols
WB protocol for YWHAH antibody 15222-1-APDownload protocol
IF protocol for YWHAH antibody 15222-1-APDownload protocol
IP protocol for YWHAH antibody 15222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

J Exp Clin Cancer Res

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Authors - Jia Song
  • KD Validated
ratWB

Eur J Pharmacol

Capsaicin prevents mitochondrial damage, protects cardiomyocytes subjected to anoxia/reoxygenation injury mediated by 14-3-3η/Bcl-2.

Authors - Jiyi Huang
humanWB

Front Cell Dev Biol

Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η.

Authors - Jiarui Liu