YWHAZ Monoclonal antibody
YWHAZ Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgM
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3G12B10
Cat no : 67698-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Product Information
67698-1-Ig targets YWHAZ in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Immunogen | YWHAZ fusion protein Ag6667 |
Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide |
Calculated molecular weight | 28 kDa |
Observed molecular weight | 28-30 kDa |
GenBank accession number | BC003623 |
Gene symbol | YWHAZ |
Gene ID (NCBI) | 7534 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.
Protocols
Product Specific Protocols | |
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WB protocol for YWHAZ antibody 67698-1-Ig | Download protocol |
IHC protocol for YWHAZ antibody 67698-1-Ig | Download protocol |
IF protocol for YWHAZ antibody 67698-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |