Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain, mouse brain |
| Positive IHC detected in | human skin cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF-Fro detected in | mouse testis tissue |
| Positive IF/ICC detected in | C2C12 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80762-1-RR targets Alpha Tubulin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag18034 Product name: Recombinant human Tubulin-Alpha protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-451 aa of BC009314 Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY Predict reactive species |
| Full Name | tubulin, alpha 1b |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC009314 |
| Gene Symbol | Alpha Tubulin |
| Gene ID (NCBI) | 10376 |
| RRID | AB_2918911 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68363 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Alpha Tubulin antibody 80762-1-RR | Download protocol |
| IHC protocol for Alpha Tubulin antibody 80762-1-RR | Download protocol |
| WB protocol for Alpha Tubulin antibody 80762-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Dose Response The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury. | ||
Am J Pathol SYT8 Drives Colorectal Cancer Progression and Immune Evasion via the SETD1A-H3K4me3 Axis. | ||
Nat Commun Mycobacterium tuberculosis modulates phosphorylation of host ATP6V1E1 to promote intracellular survival. | ||
Tissue Cell Norcantharidin induces nephrotoxicity by disrupting the ATF4/CTH/H(2)S pathway and promoting apoptosis. | ||
FASEB J Targeting DECR1 Mitigates Ferroptosis in Airway Epithelial Cells and Promotes Inflammation Resolution in Asthma. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alice (Verified Customer) (01-14-2026) | Great antibody for immunofluorescent staining of microtubules in fixed sheep ventricular myocytes. Highly specific and looks beautiful.
|
YINGJIAN (Verified Customer) (07-24-2025) | We observed consistent and robust signal intensity across multiple replicates, indicating good reproducibility.
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