YY1 Monoclonal antibody, PBS Only

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  WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

  Western blot of YY1 in various human cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 66281-1-Ig (same clone as 66281-1-PBS)

  Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66281-1-Ig (same clone as 66281-1-PBS)

  WB result of YY1 antibody (66281-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YY1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66281-1-Ig (same clone as 66281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

  Western blot of Hela cells with mouse anti-YY1 monoclonal antibody (66281-1-Ig) at various dilutions incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COS-7 using 66281-1-Ig (same clone as 66281-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IP experiment of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

  IP result of anti-YY1 (IP:66281-1-Ig, 5ug; Detection:66281-1-Ig 1:1000) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66281-1-Ig (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (66281-1-Ig, Clone: 2E11C5 ) at dilution of 1:1800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:800 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66281-1-Ig(YY1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

YY1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66281-1-PBS

Clone No.2E11C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

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Product Information

66281-1-PBS targets YY1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17732

  Product name: Recombinant human YY1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-414 aa of BC037308

  Sequence: MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEEEDDDDEDGGGGDHGGGGGHGHAGHHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGGSSSSGGGRVKKGGGKKSGKKSYLSGGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ

• Full Name: YY1 transcription factor
• Calculated Molecular Weight: 414 aa, 45 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC037308
• Gene Symbol: YY1
• Gene ID (NCBI): 7528
• RRID: AB_2881664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25490
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.