Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, HeLa cells, mouse brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HT-29 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21324-1-AP targets ZDHHC5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14891 Product name: Recombinant human ZDHHC5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 313-662 aa of BC026967 Sequence: AMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSMSYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV Predict reactive species |
| Full Name | zinc finger, DHHC-type containing 5 |
| Calculated Molecular Weight | 715 aa, 78 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC026967 |
| Gene Symbol | ZDHHC5 |
| Gene ID (NCBI) | 25921 |
| RRID | AB_10732816 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0B5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ZDHHC5 antibody 21324-1-AP | Download protocol |
| IF protocol for ZDHHC5 antibody 21324-1-AP | Download protocol |
| IHC protocol for ZDHHC5 antibody 21324-1-AP | Download protocol |
| IP protocol for ZDHHC5 antibody 21324-1-AP | Download protocol |
| WB protocol for ZDHHC5 antibody 21324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZDHHC5 Polyclonal antibody (Cat# 21324-1-AP) has 24 citations spanning journals including Nature, Molecular Cell, Science Advances, Cancer Research, Cell Death & Differentiation, Developmental Cell, Current Biology, Cell Reports, Small, Advanced Materials, Cancer Letters, Cell Death & Disease, Cell Death Discovery, Journal of Virology, Scientific Reports, Molecular Neurobiology, Biochimica et Biophysica Acta, Bioscience Reports, World Journal of Gastroenterology, Biophysical Journal, Journal of Nutritional Biochemistry, Toxicology and Applied Pharmacology, Journal of Advanced Research, and bioRxiv. Research fields cover palmitoylation, cancer, neurology, inflammation, metabolism, autophagy, and viral infection.
| Species | Application | Title |
|---|---|---|
Adv Mater Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy | ||
Cancer Res ZDHHC2-mediated AGK palmitoylation activates AKT/mTOR signaling to reduce sunitinib sensitivity in renal cell carcinoma
| ||
J Adv Res Neonatal sevoflurane exposures inhibits DHHC5-mediated palmitoylation of TfR1 in oligodendrocytes, leading to hypomyelination and neurological impairments | ||
Mol Cell ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation | ||
Sci Adv Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis |
Reviews
What customers say
The single review for this product is a 5-star rating. The customer used the antibody for Western Blot and Immunofluorescence in primary hippocampal neuron cell culture and reported that the results were consistent with expectations. Overall, the feedback is positive, indicating reliable performance across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Candice (Verified Customer) (10-30-2025) | Used for WB and IF : results are consistent with expectations.
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