ZDHHC5 Polyclonal antibody

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  WB analysis of HeLa using 21324-1-AP

  WB result of ZDHHC5 antibody (21324-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZDHHC5 transfected HeLa cells.

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  WB analysis of mouse kidney using 21324-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21324-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21324-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21324-1-AP

  IP result of anti-ZDHHC5 (IP:21324-1-AP, 4ug; Detection:21324-1-AP 1:500) with HEK-293 cells lysate 1680 ug.

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  IF Staining of HeLa using 21324-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21324-1-AP

  Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HT-29 using 21324-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 21324-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZDHHC5 (21324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

ZDHHC5 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21324-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5, Zinc finger DHHC domain-containing protein 5

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, HeLa cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: HeLa cells, HT-29 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

21324-1-AP targets ZDHHC5 in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14891

  Product name: Recombinant human ZDHHC5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 313-662 aa of BC026967

  Sequence: AMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSMSYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV

• Full Name: zinc finger, DHHC-type containing 5
• Calculated Molecular Weight: 715 aa, 78 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC026967
• Gene Symbol: ZDHHC5
• Gene ID (NCBI): 25921
• RRID: AB_10732816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C0B5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D; Authors - Gang Du
human	WB,IP	Cell Death Differ; Palmitoylation restricts SQSTM1/p62-mediated autophagic degradation of NOD2 to modulate inflammation.; Authors - Lingli Zhou ; KD Validated
human	WB	Cell Death Dis; ZDHHC8 critically regulates seizure susceptibility in epilepsy.; Authors - Qin Yang
human	WB,IP,IF	Sci Rep; DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling.; Authors - Shaymaa Mohamed Mohamed Badawy ; KD Validated
mouse	WB,CoIP	Mol Neurobiol; AKAP150 and its Palmitoylation Contributed to Pain Hypersensitivity Via Facilitating Synaptic Incorporation of GluA1-Containing AMPA Receptor in Spinal Dorsal Horn.; Authors - Yinxia Li
	WB,IF	J Virol; Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation; Authors - Qisheng Qian