ZFR Polyclonal antibody

ZFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23174-1-AP

Synonyms

hZFR, M phase phosphoprotein homolog, ZFR, ZFR1

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Validation Data Gallery

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  WB analysis using 23174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23174-1-AP (ZFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 23174-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 23174-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human small intestine tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23174-1-AP targets ZFR in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZFR fusion protein Ag19559
• Full Name: zinc finger RNA binding protein
• Calculated Molecular Weight: 1074 aa, 117 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC137085
• Gene Symbol: ZFR
• Gene ID (NCBI): 51663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.