Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25458-1-AP targets ZNF211 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22135 Product name: Recombinant human ZNF211 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 199-268 aa of BC110856 Sequence: CREIRKDFLANMRFLHQDATQTGEKPNNSNKCAVAFYSGKSHHNWGKCSKAFSHIDTLVQDQRILTREGL Predict reactive species |
| Full Name | zinc finger protein 211 |
| Calculated Molecular Weight | 576 aa, 66 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC110856 |
| Gene Symbol | ZNF211 |
| Gene ID (NCBI) | 10520 |
| RRID | AB_2880090 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13398 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF211 antibody 25458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody rates it 4 out of 5 stars for Western Blot. The reviewer used a 1:500 dilution on HEK293T and MT4 cells and noted that the antibody performs well at low dilutions but works best with PVDF membranes specifically. Overall, the feedback is positive, highlighting reliable performance under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (10-26-2022) | This antibody works at low dilutions and using PVDF only.
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