ZNF460 Polyclonal antibody

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• KD/KO Validated

ZNF460 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25299-1-AP

Synonyms

HZF8, Zinc finger protein 272, zinc finger protein 460, Zinc finger protein HZF8, ZNF272, ZNF460

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Validation Data Gallery

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  WB analysis using 25299-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25299-1-AP (ZNF460 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25299-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25299-1-AP (ZNF460 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25299-1-AP (ZNF460 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25299-1-AP (ZNF460 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF460 antibody (25299-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 25299-1-AP is ZNF460 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF460 fusion protein Ag18106
• Full Name: zinc finger protein 460
• Calculated Molecular Weight: 562 aa, 64 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC118625
• Gene Symbol: ZNF460
• Gene ID (NCBI): 10794
• RRID: AB_2880016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,human		Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB,IHC	J Cancer; Overexpression of ZNF460 predicts worse survival and promotes metastasis through JAK2/STAT3 signaling pathway in patient with colon cancer.; Authors - Tengfei Hao