ZNF740 Polyclonal antibody

ZNF740 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25411-1-AP

Synonyms

Zinc finger protein 740, Zfp740, TB7, OriLyt TD-element-binding protein 7

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Validation Data Gallery

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  WB analysis of mouse brain using 25411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25411-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 25411-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25411-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 25411-1-AP

  IP result of anti-ZNF740 (IP:25411-1-AP, 3ug; Detection:25411-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human ovary cancer using 25411-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25411-1-AP (ZNF740 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, PC-3 cells, mouse liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

25411-1-AP targets ZNF740 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF740 fusion protein Ag22295
• Full Name: zinc finger protein 740
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 22-30 kDa
• GenBank Accession Number: BC053557
• Gene Symbol: ZNF740
• Gene ID (NCBI): 283337
• RRID: AB_2880065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator; Authors - Lixia Zhang ; KD Validated
human	WB,IHC,ChIP	Int J Oncol; ZNF740 facilitates the malignant progression of hepatocellular carcinoma via the METTL3/HIF‑1A signaling axis; Authors - Hao Zhang