ZO-3 Recombinant monoclonal antibody

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• ZO-3 Recombinant monoclonal antibody (87266-1-RR)

• 

  WB analysis using 87266-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87266-1-RR (ZO-3 antibody) at a dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 87266-1-RR

  IP result of anti-ZO-3 (IP:87266-1-RR, 4ug; Detection:87266-1-RR 1:2000) with HT-29 cells lysate 1760 ug.

• 

  Affinity of 87266-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87266-1-RR against Human ZO-3 were performed. The affinity constant is 1.59 nM.

ZO-3 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 87266-1-RR

Clone No.252499G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Tight junction protein 3, Tight junction protein ZO-3, TJP3, ZO3, Zona occludens protein 3

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Tested Applications

• Positive WB detected in: HT-29 cells, HCT 116 cells, Caco-2 cells
• Positive IP detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87266-1-RR targets ZO-3 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5585

  Product name: Recombinant human ZO3/Zonula occludens PROTEIN 3 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 835-919 aa of NM_001267560.1

  Sequence: DEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL

• Full Name: tight junction protein 3 (zona occludens 3)
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_001267560.1
• Gene Symbol: ZO-3
• Gene ID (NCBI): 27134
• RRID: AB_3745435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95049-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tight junction (or zonula occludens) forms the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-3 (also known as TJP3)is a key scaffold protein of tight junctions. ZO-3 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs).ZO-3 regulates the stability of Cyclin D1, thereby influencing the proliferation of epithelial cells (PMID: 21411630).