ZRANB1 Polyclonal antibody

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• ZRANB1 Polyclonal antibody (27050-1-AP)

ZRANB1 Polyclonal Antibody for IP, ELISA

Cat No. 27050-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

hTrabid, TRABID, Ubiquitin thioesterase ZRANB1

• 

  IP experiment of HeLa using 27050-1-AP

  IP result of anti-ZRANB1 (IP:27050-1-AP, 4ug; Detection:27050-1-AP 1:500) with HeLa cells lysate 1600 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse cerebellum using 27050-1-AP

  IP result of anti-ZRANB1 (IP:27050-1-AP, 4ug; Detection:27050-1-AP 1:500) with mouse cerebellum tissue lysate 2000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IP detected in: HeLa cells, mouse cerebellum tissue

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27050-1-AP targets ZRANB1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25789

  Product name: Recombinant human ZRANB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-100 aa of AL832925

  Sequence: MSERGIKWACEYCTYENWPSAIKCTMCRAQRPSGTIITEDPFKSGSSDVGRDWDPSSTEGGSSPLICPDSSARPRVKSSYSMENANKWSCHMCTYLNWPR

• Full Name: zinc finger, RAN-binding domain containing 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: AL832925
• Gene Symbol: ZRANB1
• Gene ID (NCBI): 54764
• RRID: AB_3742213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UGI0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZRANB1 is an 81-kDa deubiquitinating enzyme with an Npl14 zinc finger (NZF) domain at the N-terminus and an ovarian tumor (OTU) domain at the C-terminus. ZRANB1 specifically cleaves K29-, K33-, and K63-linked ubiquitin chains, but a mutation of amino acid 443 from cysteine to serine ablates enzymatic activity . (PMID: 36087511, PMID: 34765294)

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Biol Ther; UCHL5 suppresses thyroid carcinoma progression via ZRANB1 stabilization and ferroptosis regulation.; Authors - Wenxia Xiao