CoraLite® Plus 488-conjugated Alpha Tubulin Monoclonal antibody
Alpha Tubulin Monoclonal Antibody for FC (Intra), IF, WB
Cat no : CL488-66031
|Positive WB detected in||HepG2 cells, HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells|
|Positive IF detected in||HeLa cells, mouse embryo tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:300-1:600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of CL488-66031 is Alpha Tubulin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, Canine|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Alpha Tubulin fusion protein Ag18034|
|Full Name||tubulin, alpha 1b|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC009314|
|Gene ID (NCBI)||10376|
|Conjugate||CoraLite® Plus 488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||493 nm / 522 nm|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
Free Radic Biol Med
Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling
A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella.
Transl Androl Urol
Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility.