Alpha Tubulin Monoclonal antibody

Alpha Tubulin Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, Canine and More (7)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E4C11

Cat no : 66031-1-Ig

Synonyms

alpha Tubulin, Alpha tubulin ubiquitous, TUBA1B, tubulin, Tubulin alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin


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  • Western Blot (WB) analysis of various lysates using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    WB analysis using 66031-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IP experiment of HeLa using 66031-1-Ig

    IP result of anti-Alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human tonsillitis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human colon cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human colon cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human liver cancer using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IHC staining of human tonsillitis using 66031-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HeLa using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Histone H1.2 antibody (19649-1-AP, green).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HepG2 using 66031-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of MCF-7 using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZO-1 antibody (21773-1-AP, green).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of MCF-7 using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ZO-1 antibody (21773-1-AP, red).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of rat brain using 66031-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    IF Staining of HepG2 using 66031-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Alpha Tubulin Monoclonal antibody (66031-1-Ig)

    FC experiment of HeLa using 66031-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

"Alpha Tubulin Antibodies" Comparison

View side-by-side comparison of Alpha Tubulin antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, rat brain tissue, MCF-7 cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66031-1-Ig targets Alpha Tubulin in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, goat, tick, chicken, sheep, monkey, rabbit, zebrafish
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034
Full Name tubulin, alpha 1b
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC009314
Gene Symbol TUBA1B
Gene ID (NCBI) 10376
RRIDAB_11042766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).


Protocols

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
IHC protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
IF protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
IP protocol for Alpha Tubulin antibody 66031-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

Structural basis for FGF hormone signalling

Authors - Lingfeng Chen
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
human,monkeyWB,IF

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
humanWB

Signal Transduct Target Ther

FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth

Authors - Wenxia Wei
humanWB

Nat Microbiol

PMID: 31649358

Authors - Tingting Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alexandra (Verified Customer) (03-07-2024)

Extremely efficient!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000x
  • Cell Tissue Type: U2OS human osteosarcoma cell line
Alpha Tubulin Antibody Western Blot validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:66031-1-Ig)
FH

Chiara (Verified Customer) (10-02-2023)

This antibody worked quite well in our immunofluorescence experiments

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human primary skin fibroblasts
Alpha Tubulin Antibody Immunofluorescence validation (1:1000 dilution) in human primary skin fibroblasts (Cat no:66031-1-Ig)
FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB at 1:2000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Bone Marrow MSCs
FH

Amy (Verified Customer) (08-10-2023)

Clean strong bands for western blot on HEK293T lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:50,000
  • Cell Tissue Type: HEK293T
FH

Kalin (Verified Customer) (03-12-2022)

I think this antibody works great for western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Sea urchin lysate
FH

Silvia (Verified Customer) (02-18-2022)

This antibody worked quite well in our western blotting and it comes at a great price.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa
FH

Azita (Verified Customer) (06-02-2021)

Immunohistochemistry labelling of (4% PFA) fixed mice spinal cord tissues using Alpha Tubulin Monoclonal Antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mice spinal cord tissues
FH

Scooby (Verified Customer) (10-05-2020)

happy

  • Applications: Immunofluorescence
  • Cell Tissue Type: n/a
FH

Lauren (Verified Customer) (10-05-2020)

thanks

  • Applications: Western Blot
FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2,000
  • Cell Tissue Type: HeLa cells