citrate synthase Monoclonal antibody

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  WB analysis using 67784-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67784-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67784-1-Ig (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67784-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67784-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse breast cancer using 67784-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of MCF-7 using 67784-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

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  IF Staining of HepG2 using 67784-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

citrate synthase Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, ELISA

Cat No. 67784-1-Ig

Clone No.2F9G6

Publications(2)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, ELISA

Synonyms

CS, 2F9G6, CISY, Citrate, Citrate synthase, mitochondrial

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse breast cancer
• Positive IF/ICC detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67784-1-Ig targets citrate synthase in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: citrate synthase fusion protein Ag9255
• Full Name: citrate synthase
• Calculated Molecular Weight: 466 aa, 52 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC010106
• Gene Symbol: Citrate Synthase
• Gene ID (NCBI): 1431
• RRID: AB_2918548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75390
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

Publications

Species	Application	Title
human	WB	Cell Death Dis; BNIP3-mediated mitophagy boosts the competitive growth of Lenvatinib-resistant cells via energy metabolism reprogramming in HCC; Authors - Sikai Wang
mouse	WB	Aging Cell; Ketogenic β-hydroxybutyrate regulates β-hydroxybutyrylation of TCA cycle-associated enzymes and attenuates disease-associated pathologies in Alzheimer's mice; Authors - Wanhong Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (02-21-2023)

It works very well in adipocytes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes