CoraLite® Plus 488-conjugated citrate synthase Monoclonal antibody
citrate synthase Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F9G6
Cat no : CL488-67784
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-67784 targets citrate synthase in IF applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | citrate synthase fusion protein Ag9255 |
Full Name | citrate synthase |
Calculated molecular weight | 466 aa, 52 kDa |
GenBank accession number | BC010106 |
Gene symbol | CS |
Gene ID (NCBI) | 1431 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 citrate synthase antibody CL488-67784 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |