CoraLite® Plus 647-conjugated citrate synthase Monoclonal antibody

citrate synthase Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2F9G6

Cat no : CL647-67784

Synonyms

citrate synthase, CS

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Validation Data Gallery

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  IF Staining of MCF-7 using CL647-67784

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 647 citrate synthase antibody (CL647-67784, Clone: 2F9G6 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67784 targets citrate synthase in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: citrate synthase fusion protein Ag9255
• Full Name: citrate synthase
• Calculated Molecular Weight: 466 aa, 52 kDa
• GenBank Accession Number: BC010106
• Gene Symbol: CS
• Gene ID (NCBI): 1431
• RRID: AB_2920310
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.