Filter: AllWBIHCIF-FroIF/ICC

WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.
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LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.
WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67784-1-Ig (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.
IF Staining of mouse breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67784-1-Ig (same clone as 67784-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 67784-1-Ig (same clone as 67784-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

citrate synthase Monoclonal antibody, PBS Only

citrate synthase Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

Cat No. 67784-1-PBS

Clone No.2F9G6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

Synonyms

CS, 2F9G6, CISY, Citrate, Citrate synthase, mitochondrial

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67784-1-PBS targets citrate synthase in WB, IHC, IF/ICC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag9255 Product name: Recombinant human CS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 132-466 aa of BC010106 Sequence: EQVSWLSKEWAKRAALPSHVVTMLDNFPTNLHPMSQLSAAVTALNSESNFARAYAQGISRTKYWELIYEDSMDLIAKLPCVAAKIYRNLYREGSGIGAIDSNLDWSHNFTNMLGYTDHQFTELTRLYLTIHSDHEGGNVSAHTSHLVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKIVPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPKSMSTEGLMKFVDSKSG Predict reactive species
Full Name	citrate synthase
Calculated Molecular Weight	466 aa, 52 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC010106
Gene Symbol	Citrate Synthase
Gene ID (NCBI)	1431
RRID	AB_2918548
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O75390
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.