Cyclin T2 Polyclonal antibody, PBS Only

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  WB analysis using 21860-1-AP (same clone as 21860-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21860-1-AP (cyclin T2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21860-1-AP (same clone as 21860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 4010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21860-1-AP (same clone as 21860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21860-1-AP (same clone as 21860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

Cyclin T2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21860-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CCNT2, Cyclin T, Cyclin-T2, CycT2

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Product Information

21860-1-PBS targets Cyclin T2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16514

  Product name: Recombinant human CCNT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 251-610 aa of BC114366

  Sequence: LKKIRNWRANQAARKPKVDGQVSETPLLGSSLVQNSILVDSVTGVPTNPSFQKPSTSAFPAPVPLNSGNISVQDSHTSDNLSMLATGMPSTSYGLSSHQEWPQHQDSARTEQLYSQKQETSLSGSQYNINFQQGPSISLHSGLHHRPDKISDHSSVKQEYTHKAGSSKHHGPISTTPGIIPQKMSLDKYREKRKLETLDLDVRDHYIAAQVEQQHKQGQSQAASSSSVTSPIKMKIPIANTEKYMADKKEKSGSLKLRIPIPPTDKSASKEELKMKIKVSSSERHSSSDEGSGKSKHSSPHISRDHKEKHKEHPSSRHHTSSHKHSHSHSGSSSGGSKHSADGIPPTVLRSPVGLSSDGI

• Full Name: cyclin T2
• Calculated Molecular Weight: 730 aa, 81 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC114366
• Gene Symbol: Cyclin T2
• Gene ID (NCBI): 905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60583
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cyclin T2 (CCNT2) belongs to the cyclin family. CCNT2 is regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II)  (PMID: 9499409, PMID: 15563843). Cyclin T2 is expressed at a relatively high level in freshly isolated monocytes and its level remains constant when the cells are induced to undergo macrophage differentiation (PMID: 21791050). In contrast, Cyclin T1 is expressed at low levels in monocytes and it is strongly up-regulated by a post-transcriptional mechanism when the cells are induced to differentiate to macrophages.