SpeedyPLEX CBA Assay Panels

SpeedyPLEX Kits utilize fluorescent dye, color-coded beads which are conjugated to capture antibodies. The capture beads are incubated together with sample and corresponding biotinylated detection antibodies to assemble the sandwich complex. Streptavidin-PE is then added, which is used for detecting the concentration of target analytes via flow cytometry. Through this simple workflow, samples can be prepared for analysis in as little as 90 minutes.

Proteintech SpeedyPLEX Kits work on any standard flow cytometer equipped with two lasers (488/532 nm and 635 nm) for PE and APC detection.

Analysis of cell supernatants collected from human PBMCs that stimulated with 10ug/mL PHA for 3 days. Supernatants were assayed for IFN-gamma, IL-6, IL-10, IL-1 alpha, and TNF alpha using the Human Cytokine (4-plex) SpeedyPLEX Kit 2 (KMF00002) followed by data analysis on Beckman Coulter® CytoFLEX (blue), Agilent® NovoCyte (light blue), and Challenbio FongCyteTM (green) flow cytometers. The measured analyte concentration is comparable across all cytometers with no noticeable differences seen.

Compared to traditional, single analyte ELISAs, cytometric bead arrays provide several advantages

Feature	Cytometric Bead Arrays	Traditional ELISA
Sample Utilization	Multiple analytes from as little as 25 µL	100–200 µL per single target to account for duplicate/triplicate and possible dilution
Dynamic Range	Detects multiple analytes at varying concentrations	Limited to single analyte per well
Data Variability	Lower variability via simultaneous measurement	Higher plate-to-plate variation
Cost Per Analyte	Lower — multiplex from a single sample	Higher — separate plate needed per analyte
Throughput	Multiple analytes in a single run, more high-throughput	One analyte per plate, multiple runs needed