gp130/IL6ST Monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP50086-1

  Sandwich ELISA standard curve of MP50086-1, gp130/IL6ST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68692-1-PBS. Detection antibody: 68692-2-PBS. Standard: Eg0654. Range: 31.25-2000 pg/mL.

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  Sample test of MP50086-1

  Serum of sixteen individual healthy human donors was measured. The human gp130/IL6ST concentration of detected samples was determined to be 241.00 ng/mL with a range of 67.75 - 450.06 ng/mL.

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  Sample test of MP50086-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean gp130/IL6ST concentration was determined to be 248.5 pg/mL in unstimulated PBMC supernatant, 754.8 pg/mL in PHA stimulated PBMC supernatant.

gp130/IL6ST Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68692-1-PBS

Clone No.1A2A1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL6ST, CD130, Glycoprotein 130, GP130, GP130 RAPS

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Product Information

68692-1-PBS targets gp130/IL6ST as part of a matched antibody pair:

MP50086-1: 68692-1-PBS capture and 68692-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0654

  Product name: Recombinant Human gp130/IL6ST protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 23-618 aa of NM_002184.3

  Sequence: ELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTIINRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVEWTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYADGPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTIIGNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEI

• Full Name: interleukin 6 signal transducer (gp130, oncostatin M receptor)
• Calculated Molecular Weight: 104 kDa
• GenBank Accession Number: NM_002184.3
• Gene Symbol: IL6ST
• Gene ID (NCBI): 3572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P40189-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.