hCG Alpha Polyclonal antibody

hCG Alpha Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 25014-1-AP

Synonyms

CG ALPHA, CGA, Choriogonadotropin alpha chain, Follitropin alpha chain, FSH alpha, FSHA, GPHa, GPHA1, HCG, hCG alpha, LHA, LSH alpha, Lutropin alpha chain, Thyrotropin alpha chain, TSH alpha, TSHA

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Validation Data Gallery

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  WB analysis using 25014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25014-1-AP (hCG alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 25014-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25014-1-AP (hCG alpha antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25014-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25014-1-AP (hCG Alpha antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 25014-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25014-1-AP (hCG Alpha antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: JAR cells, human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

25014-1-AP targets hCG Alpha in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: hCG Alpha fusion protein Ag17313
• Full Name: glycoprotein hormones, alpha polypeptide
• Calculated Molecular Weight: 116 aa, 13 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC020782
• Gene Symbol: hCG Alpha
• Gene ID (NCBI): 1081
• RRID: AB_2879846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
human	IHC	J Clin Endocrinol Metab; A Patient with a Thyrotropin-secreting Microadenoma and Resistance to Thyroid Hormone (P453T).; Authors - Xiaochun Teng
human	WB	iScience; L3MBTL2-mediated CGA transcriptional suppression promotes pancreatic cancer progression through modulating autophagy.; Authors - Hua Huang
human	IHC	Front Endocrinol (Lausanne); Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment; Authors - Yunpeng Wang
human	IHC	J Biol Chem; miR-107 reverses the multidrug resistance of gastric cancer by targeting the CGA/EGFR/GATA2 positive feedback circuit; Authors - Pei Wang