hCG Alpha Polyclonal antibody

hCG Alpha Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25014-1-AP

Synonyms

CG ALPHA, CGA, Choriogonadotropin alpha chain, Follitropin alpha chain, FSH alpha, FSHA, GPHa, GPHA1, HCG, hCG alpha, LHA, LSH alpha, Lutropin alpha chain, Thyrotropin alpha chain, TSH alpha, TSHA



Tested Applications

Positive WB detected inJAR cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 25014-1-AP is hCG Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen hCG Alpha fusion protein Ag17313
Full Name glycoprotein hormones, alpha polypeptide
Calculated molecular weight 116 aa, 13 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC020782
Gene symbol CGA
Gene ID (NCBI) 1081
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for hCG Alpha antibody 25014-1-APDownload protocol
IHC protocol for hCG Alpha antibody 25014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Clin Endocrinol Metab

A Patient with a Thyrotropin-secreting Microadenoma and Resistance to Thyroid Hormone (P453T).

Authors - Xiaochun Teng