PCBP1 Polyclonal antibody, PBS Only

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  WB analysis using 14523-1-AP (same clone as 14523-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 14523-1-AP (same clone as 14523-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14523-1-AP (same clone as 14523-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 14523-1-AP (same clone as 14523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14523-1-AP (same clone as 14523-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 14523-1-AP (same clone as 14523-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 14523-1-AP (same clone as 14523-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14523-1-AP (same clone as 14523-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 14523-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PCBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14523-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hnRNP E1, hnRNP E1/PCBP1, hnRNP-E1, Alpha CP1, Alpha-CP1

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Product Information

14523-1-PBS targets PCBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6007

  Product name: Recombinant human hnRNP-E1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-356 aa of BC039742

  Sequence: MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

• Full Name: poly(rC) binding protein 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC039742
• Gene Symbol: PCBP1
• Gene ID (NCBI): 5093
• RRID: AB_2160619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15365
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.