Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, MDA-MB-231 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, NIH/3T3 cells, A431 cells, sodium arsenite treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14523-1-AP targets PCBP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6007 Product name: Recombinant human hnRNP-E1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-356 aa of BC039742 Sequence: MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS Predict reactive species |
| Full Name | poly(rC) binding protein 1 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC039742 |
| Gene Symbol | PCBP1 |
| Gene ID (NCBI) | 5093 |
| RRID | AB_2160619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15365 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCBP1 antibody 14523-1-AP | Download protocol |
| IHC protocol for PCBP1 antibody 14523-1-AP | Download protocol |
| IP protocol for PCBP1 antibody 14523-1-AP | Download protocol |
| WB protocol for PCBP1 antibody 14523-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
14523-1-AP is an anti-PCBP1 antibody with 23 citations. Publications appear in journals including Cell, Signal Transduction and Targeted Therapy, Autophagy, Molecular Cell, Nucleic Acids Research, Journal of Clinical Investigation, Advanced Science, Cancer Letters, and Cell Reports. Research fields span ferroptosis, cancer biology (colorectal, pancreatic, esophageal, lung, and hepatocellular carcinoma), RNA biology and splicing, stem cell biology, neurodegeneration, cardiovascular disease, immunology, and virology, with applications in WB, IHC, IF, and FC.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Acevaltrate as a novel ferroptosis inducer with dual targets of PCBP1/2 and GPX4 in colorectal cancer | ||
Autophagy Mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via a PIEZO1-NCOA4-ZBP1 axis. | ||
Mol Cell Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. | ||
Nucleic Acids Res G-Quadruplex binding enantiomers show chiral selective interactions with human telomere. | ||
J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used it for Western Blot at a 1:1000 dilution on 293T cells and reported satisfaction with the results obtained. Overall, the feedback indicates reliable performance and good outcomes in the tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S. H. (Verified Customer) (02-24-2020) | we are happy for the result we got.
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