hnRNP-E1 Polyclonal antibody

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  WB analysis using 14523-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14523-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14523-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14523-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14523-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14523-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14523-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 14523-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of A431 using 14523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

hnRNP-E1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14523-1-AP

Publications(16)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

hnRNP E1/PCBP1, hnRNP E1, PCBP1, Nucleic acid-binding protein SUB2.3, HNRPE1

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, NIH/3T3 cells, A431 cells, sodium arsenite treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

14523-1-AP targets hnRNP-E1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6007

  Product name: Recombinant human hnRNP-E1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-356 aa of BC039742

  Sequence: MDAGVTESGLNVTLTIRLLMHGKEVGSIIGKKGESVKRIREESGARINISEGNCPERIITLTGPTNAIFKAFAMIIDKLEEDINSSMTNSTAASRPPVTLRLVVPATQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGVPQSVTECVKQICLVMLETLSQSPQGRVMTIPYQPMPASSPVICAGGQDRCSDAAGYPHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGFAGIDSSSPEVKGYWASLDASTQTTHELTIPNNLIGCIIGRQGANINEIRQMSGAQIKIANPVEGSSGRQVTITGSAASISLAQYLINARLSSEKGMGCS

• Full Name: poly(rC) binding protein 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC039742
• Gene Symbol: PCBP1
• Gene ID (NCBI): 5093
• RRID: AB_2160619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15365
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
human	IF	Nucleic Acids Res; G-Quadruplex binding enantiomers show chiral selective interactions with human telomere.; Authors - Jiasi Wang
human	IHC	Bioengineered; PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma.; Authors - Kaiming Peng
rat	WB,IHC	Pathol Int; PCBP2 knockdown promotes ferroptosis in malignant mesothelioma.; Authors - Lin Yue ; KD Validated
mouse	WB	Mol Cell; Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.; Authors - David Colognori
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S. H. (Verified Customer) (02-24-2020)

we are happy for the result we got.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000x dilution
• Cell Tissue Type: 293T CELLS

hnRNP-E1 Antibody Western Blot, validation (1000x dilution dilution) in 293T CELLS (Cat no:14523-1-AP)