human IgM Polyclonal antibody, PBS Only

• 

  WB analysis of human plasma using 11016-1-AP (same clone as 11016-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 11016-1-AP (IGHM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11016-1-AP (same clone as 11016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11016-1-AP (same clone as 11016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

human IgM Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11016-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

IgM, IGHM, VH, MU

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Product Information

11016-1-PBS targets human IgM in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1459

  Product name: Recombinant human IGHM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC009851

  Sequence: FGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLWATASTFIVLFLLSLFYSTTVTLFKVK

• Full Name: immunoglobulin heavy constant mu
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC009851
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• RRID: AB_593462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa).