human IgM Antibody

human IgM Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11016-1-AP

Synonyms

IGHM, MU, VH



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11016-1-AP targets human IgM in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
Observed molecular weight 75 kDa
GenBank accession number BC009851
Gene symbol IGHM
Gene ID (NCBI) 3507
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa).

Protocols

Product Specific Protocols
WB protocol for human IgM antibody 11016-1-APDownload protocol
IHC protocol for human IgM antibody 11016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols