DCTN1/p150 glued Polyclonal antibody, PBS Only

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  WB analysis of human brain using 55182-1-AP (same clone as 55182-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55182-1-AP (same clone as 55182-1-PBS)

  WB result of DCTN1/p150 glued antibody (55182-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCTN1/p150 glued transfected HeLa cells. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55182-1-AP (same clone as 55182-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 55182-1-AP (same clone as 55182-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DCTN1/p150 glued Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 55182-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DCTN1, p150 glued, DP-150, DCTN 1, DAP-150

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Product Information

55182-1-PBS targets DCTN1/p150 glued in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: dynactin 1 (p150, glued homolog, Drosophila)
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 135 kDa, 150 kDa
• GenBank Accession Number: NM_004082
• Gene Symbol: p150 glued
• Gene ID (NCBI): 1639
• RRID: AB_10837074
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14203
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.