p150 glued Polyclonal antibody

p150 glued Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55182-1-AP

Synonyms

DAP 150, DCTN1, DP 150, Dynactin subunit 1, HMN7B, P135, p150 glued



Tested Applications

Positive WB detected inhuman brain tissue, Jurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55182-1-AP targets p150 glued in WB, IHC, IF,ELISA applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Cited Reactivity mussel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dynactin 1 (p150, glued homolog, Drosophila)
Calculated molecular weight 142 kDa
Observed molecular weight 135 kDa, 150 kDa
GenBank accession numberNM_004082
Gene symbol DCTN1
Gene ID (NCBI) 1639
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.

Protocols

Product Specific Protocols
WB protocol for p150 glued antibody 55182-1-APDownload protocol
IHC protocol for p150 glued antibody 55182-1-APDownload protocol
IF protocol for p150 glued antibody 55182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
musselWB

Ecotoxicol Environ Saf

Proteomic profile in the mussel Perna viridis after short-term exposure to the brown tide alga Aureococcus anophagefferens.

Authors - Jian-Wei Zheng